A behind-the-scenes look at how we created our 2013 Apoptosome Assembly animation for Cell Signaling Technologies, with step-by-step tutorials that cover key concepts including research and pre-production, construction of complete protein models from available 3D structural data as well as missing data, and compositing techniques used to avoid split-attention issues.
LEARNING OBJECTIVES
- Finding structural data in the Protein Data Bank and using the data to
reconstruct complex 3D models in Maya & Molecular Maya. - Finding structural data in the Protein Data Bank and using the data to reconstruct complex 3D models in Maya & Molecular Maya.
- Dealing with incomplete or controversial scientific information
- Learning how to rig and animate multi-domain protein models
- Using compositing techniques to increase clarity and viewer’s learning experience
TABLE of CONTENTS
01 Introduction
01:10
02 Apoptosome Formation: The Main Actors
01:54
03 Searching the PDB for Apaf-1 (Molecule of the Month)
01:49
04 Finding Relevant Information on the PDB Summary Page
02:08
05 Finding Missing Structural Elements Using the PDB Protein Feature View
03:13
06 Searching the PDB for Caspase-3
01:48
07 Importing Data into Maya using Molecular Maya
02:37
08 Preparing a Structure for Dynamic Motion: Introduction to Chimera
01:30
09 Editing Structural Data Using Chimera
03:43
10 Importing Custom PDB Files into mMaya and Cleaning Up Mesh Nodes
03:03
11 Continuing the Reconstruction of Apaf-1: Part 1
02:14
12 Continuing the Reconstruction of Apaf-1: Part 2
03:30
13 What to Do When There is No Structural Data Available
02:20
14 Creating a Smooth Linker
03:21
15 Creating a Meshed Protein Cloud Style Linker
00:35
16 Finishing the Activated Apaf-1 Model
01:50
17 Building Caspase-9
04:03
18 Importing Caspase-3
01:05
19 Animating Apaf-1 Protein Folding: Part 1
02:15
22 Compositing Tricks to Avoid Spilt Attention Problems
01:41
23 After Effects: Time Remapping
03:21
24 After Effects: Editing Layer Saturation
02:10
25 Conclusion
0:40
RUN-TIME 0:57:47