Single molecular imaging, nanometry, 3D single particle tracking. Topics include: diffusional movements of single membrane proteins, 3D tracking of vesicle transport, signal transduction, chemotaxis. Lab website: visuals, methods, links to references.
Important information is generally lost by conventional ensemble averaging methods. Thus, experiments on single molecules are necessary to elucidate molecular properties and mechanisms. Work/movies from individual laboratories below.
Ueda, Masahiro
Single-molecule imaging. Topics include: cell migration, chemotaxis, signal transduction, transcription. Lab website: movies, links to references.
van Oijen, Antoine
Novel single molecule biophysics techniques. Topics include: DNA replication, viral fusion, membrane transporters. Lab website: links to references.
Xie, Xiaoliang Sunney
Single molecule spectroscopy, microscopy and enzymology. Topics include: gene expression, single cell genomics / sequencing. Lab website: structure / function analysis, visuals, methods, links to references.
Gonzalez, Ruben
Single-molecule biophysical approaches to investigate the molecular mechanism of protein synthesis. Lab website: visuals, methods, links to references.
Spudich, James
Myosin motor proteins. Lab website: Movies and links to publications about myosin motor proteins.
Block, Steven M
Kinesin motor proteins. Lab website: Movies and analysis of single molecule experiments on kinesins, links to publications.
Croquette, Vincent. Bensimon, David
Single molecule micromanipulation, in vivo single molecule fluorescence, single-cell physiology. Topics include: helicases, topoisomerases, translocases, DNA-protein interactions, chromatin. Lab website: visuals, methods, links to references.
Marriott, Gerard
Optical probes and biosensors in vitro and in vivo, super-resolution imaging. Topics include: cytoskeleton, cell motility. Lab website: visuals, movies, methods, links to references.
Kapanidis, Achillefs N
Mechanisms of gene expression using single-molecule biophysical methods; observation of single biomolecules in real time, both “in vitro” and in living cells. Topics include: DNA Polymerase, RNA Polymerase, DNA recombination, DNA biosensors and nanodevices, super-resolution imaging of bacterial replication and transcription. Lab website: structure / function analysis, visuals, methods, links to references.
Stone, Michael
Single-molecule biophysical methods to probe enzyme structure and dynamics of protein-nucleic acid complexes. Topics include: telomerase dynamics, telomeres, ribonucleoprotein (RNP) complex structure. Lab website: links to references.
Scheuring, Simon
Atomic force microscopy (AFM) based research of membrane phenomena. Topics include: structure, dynamics, diffusion, interaction, mechanics and supramolecular assembly of membrane proteins and other membrane constituents. Lab website: links to references.
Moerner, William E
Super-resolved fluorescence microscopy. Topics include: Nav Channel, GroEL, Lambda-DNA, MreB actin homolog, MHC II. Lab website: movies, links to references.